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Courage + Khazaka electronic GmbH

Invitro Tewameter® VT 310

Probe Perfectly Suited for TEWL-Measurements on Franz Cells

With the Invitro Tewameter® VT 310 a probe for the TEWL measurement is available, which offers all benefits of the Tewameter® and is perfectly suited to sit on a Franz cell.

  • Measuring with the Invitro Tewameter®

    VT probe

    The probe Tewameter® VT 310 emulates completely the upper part (donor chamber) of a Franz cell (standard is 15 mm Ø, other sizes on request). Thus the probes sits exactly on the membrane of the Franz cell as it would sit on skin directly without needing further adapters. The measured TEWL-value is expressed in g/h/m², the worldwide acknowledged absolute measuring value for skin.

    No other probe offers this possibility.

    VT with centerpiece

    If needed, the probe can also be used on a centerpiece of high quality Teflon, which fits exactly on the probe and the receptor chamber of the Franz cell without further fixing adapters. The thus measured TEWL has to be regarded relatively (no longer absolute figures as the distance between probe and membrane is changed).

    In vitro TEWL measurements have a variety of different applications:

    • Inexpensive pre-screening before in vivo tests (no ethic commission, no expensive volunteers)
    • Only way to study skin permeability and dermal absorption necessary for safety and efficacy testing
    • In vitro tests and procedures to minimize the use of animals in tests are established in several worldwide acknowledged guidelines: e.g. ECVAM (European Centre for the Validation of Alternatives to Animal Testing), OECD (Organisation for Economic Co-operation and Development) or REACH (Registration, Evaluation, Authorisation and Restriction of Chemicals)
  • Adantages of the Probe

    • The probe offers all advantages of the “open chamber” measurement of the Tewameter®, the worldwide most used TEWL-measuring device (even in space, the probe had been used for measurements on the ISS). Hundreds of publications performed with the probe prove this fact.
    • The Invitro Tewameter® probe can replace the donor chamber of a Franz cell completely. Thus it sits perfectly on the membrane, fully comparable to in vivo measurements. The results are expressed in absolute figures (TEWL = g/h/m²).
    • No further inconvenient adapters are needed.
    • If preparations are applied to the membrane during the measurement, a special centerpiece emulating the donor chamber made out of high quality, material-resistent Teflon can be put between probe and membrane. The probe fits exactly to the centerpiece and further adapters are not needed.
    • The standard probe fits to a Franz cell of 15 mm Ø. Other sizes between 10 - 20 mm Ø can be custom made (smaller or larger sizes on request).
    • Up to 10 probes can measure simultaneously.
    • Continuous measurements over very long time periods are possible
    • During the measurement all TEWL values, averages and the standard deviations as well as the information on temperature and relative humidity of the two sensor pairs in the probe can be recorded and displayed.
    • With the open chamber measurement even higher water loss values can be detected accurately as no water is collected inside the probe.
    • Accuracy of the probe can be checked anytime quickly.VT on skin
    • As the probe is very light and convenient, it may also be considered for other applications (e.g. fixing the probe to the skin with double sided sticking rings for long-term measurements, measurements under movement, etc.).
    • The Invitro Tewameter® VT 310 can be connected to the MPA-systems (please see details in Basic Probe Systems). Please ask for package prices for several Invitro Tewameter®-probes with basic unit.

    Tewitro01Other related probes available:

    • Tewitro® TW 24: TEWL measurement on up to 24 cultured skin wells simultaneously
    • Tewameter® TM Hex: the ultimative TEWL measurement with one head
    • Tewameter® TM Nano: ideal tool for small or difficult to reach skin sites (e.g. nail, lips, scalp with hair)
    • Tewameter® Triple TM 330T: TEWL measurement probe with three flexible measurement heads to measure either on a large surface or on three different surfaces simultaneously
  • Measurement Principle

    Tewameter principleThe open chamber measurement of the Tewameter® is the only method to assess the TEWL continuously, which is necessary for most applications without influencing the skin surface. Numerous studies are available.

  • Technical Data

    Probe only:
    Dimensions: Length: 6.5 cm, Measuring chamber: Height: 2 cm, Inner Ø: 1.5 cm, Outer Ø: 3 cm, Cable length: 1.3 m, Weight: approx. 60 g

    Teflon-centerpiece: Height:  2.5 cm, Inner Ø: 1.5 cm, Outer Ø: 3 cm
    These data apply to the standard probe for a 15 mm Ø Franz cell, for customized sizes they will partly vary.

    Resolution: relative humidity ± 0.01 % RH, Temp.: ± 0.01 °C, TEWL: 0.1 g/h/m², Measuring area: TEWL 0-320 g/h/m²

    Operating conditions: T: 10-40° C, RH: 30-70 % RH

    Measurement Uncertainty: for 10° C to 40° C and TEWL-values lower than 70 g/h/m²:

    TEWL: ± 10 % of the measured value plus ±1 g/h/m² for RH between 5 % and 90 %,  ± 15 % of the measured value plus ± 2 g/h/m² outside this range

    Temperature (T): ± 0.2 °C for 10 °C to 60 °C and ± 0.5 °C outside this range

    Relative humidity (RH): ± 2.0 % for 5 to 90 % RH and 10 °C to 60 °C, ± 3.0 % outside these ranges

    Technical changes may be made without prior notice.

Courage+Khazaka electronic GmbH
Mathias-Brüggen-Str. 91
50829 Köln, Germany
Phone: +49-221-9564990
Fax: +49-221-9564991
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